{"id":434558,"date":"2026-08-24T17:24:54","date_gmt":"2026-08-24T09:24:54","guid":{"rendered":"https:\/\/library.usj.edu.mo\/?post_type=tnc_col_19837_item&p=434558"},"modified":"2026-08-24T18:31:24","modified_gmt":"2026-08-24T10:31:24","slug":"comparative-materials-level-evaluation-of-3%e2%80%b2-and-5%e2%80%b2-thiol-dna-aptamer-conjugation-on-gold-nanospheres-and-nanoflowers-apparent-dna-loading-output-morphology-retention-and-qualitati","status":"publish","type":"tnc_col_19837_item","link":"https:\/\/library.usj.edu.mo\/zh\/scholarly-work-usj\/comparative-materials-level-evaluation-of-3%e2%80%b2-and-5%e2%80%b2-thiol-dna-aptamer-conjugation-on-gold-nanospheres-and-nanoflowers-apparent-dna-loading-output-morphology-retention-and-qualitati\/","title":{"rendered":"COMPARATIVE MATERIALS-LEVEL EVALUATION OF 3\u2032- AND 5\u2032-THIOL DNA APTAMER CONJUGATION ON GOLD NANOSPHERES AND NANOFLOWERS: APPARENT DNA LOADING OUTPUT, MORPHOLOGY RETENTION, AND QUALITATIVE SALT-CHALLENGE RESPONSE"},"content":{"rendered":"

GOLD NANOSPHERES (AUNPS) AND GOLD NANOFLOWERS (AUNFS) ARE WIDELY USED AS PLATFORMS FOR DNA APTAMER FUNCTIONALIZATION, WHILE CONJUGATION BEHAVIOR AND COLLOIDAL TOLERANCE REMAIN IMPORTANT FACTORS AFFECTING SUBSEQUENT SENSING-ORIENTED OPTIMIZATION. IN THIS STUDY, 82-NT THIOLATED DNA APTAMER CONSTRUCTS BEARING EITHER 3\u2032-SH OR 5\u2032-SH TERMINAL MODIFICATION WERE IMMOBILIZED ONTO CITRATE-STABILIZED AUNPS AND AUNFS UNDER MATCHED STEPWISE SALT-AGING CONDITIONS. APPARENT NANOPARTICLE-ASSOCIATED DNA OUTPUT WAS ESTIMATED BY QUBIT-BASED MEASUREMENT OF UNBOUND SSDNA IN THE SUPERNATANT AND EXPRESSED AS MASS-BASED LOADING OUTPUT (NG). UNDER THE TESTED STOCK-DISPERSION CONDITIONS, AUNP SAMPLES SHOWED HIGHER APPARENT CONJUGATION OUTPUT THAN AUNF SAMPLES. SPECIFICALLY, THE APPARENT CONJUGATION YIELDS FOR AUNPS WERE 80.65 \u00b1 1.64% (3\u2032-SH) AND 84.76 \u00b1 1.98% (5\u2032-SH), WHEREAS THOSE FOR AUNFS WERE 66.64 \u00b1 3.36% (3\u2032-SH) AND 73.65 \u00b1 1.36% (5\u2032-SH). THE CORRESPONDING APPARENT DNA LOADING OUTPUTS WERE 2329.7 \u00b1 47.4 NG AND 2448.7 \u00b1 57.1 NG FOR AUNPS, AND 1925.1 \u00b1 97.0 NG AND 2127.4 \u00b1 39.3 NG FOR AUNFS. DLS SIZE INCREASES AND ZETA POTENTIAL SHIFTS TOWARD MORE NEGATIVE VALUES WERE CONSISTENT WITH THE FORMATION OF A DNA-ASSOCIATED INTERFACIAL LAYER, WHILE TEM IMAGES SUPPORTED MORPHOLOGY RETENTION AFTER CONJUGATION. A QUALITATIVE VISUAL SALT-CHALLENGE ASSESSMENT INDICATED THAT APTAMER-FUNCTIONALIZED NANOPARTICLES DISPLAYED IMPROVED RESISTANCE TO SALT-INDUCED AGGREGATION RELATIVE TO BARE PARTICLES UNDER THE TESTED CONDITIONS. BECAUSE THE COMMERCIALLY SUPPLIED AUNP AND AUNF DISPERSIONS WERE NOT NORMALIZED TO IDENTICAL PARTICLE NUMBER OR ACCESSIBLE SURFACE AREA, THE REPORTED VALUES SHOULD BE INTERPRETED AS COMPARATIVE APPARENT OUTPUTS RATHER THAN INTRINSIC LOADING CAPACITIES. WITHIN THIS SCOPE, THE PRESENT STUDY PROVIDES A CONVENIENT PRELIMINARY MATERIALS-LEVEL EVALUATION OF THIOLATED APTAMER CONJUGATION BEHAVIOR AND MAY SUPPORT FUTURE GLYPHOSATE APTASENSOR OPTIMIZATION.<\/p>","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","format":"standard","class_list":["post-434558","tnc_col_19837_item","type-tnc_col_19837_item","status-publish","format-standard","hentry","tnc_tax_19818-english","tnc_tax_19716-institute-of-science-and-environment","tnc_tax_19659-journal-article"],"taxonomy_info":[],"featured_image_src_large":false,"author_info":[],"comment_info":0,"featured_img":false,"coauthors":[],"author_meta":{"author_link":"https:\/\/library.usj.edu.mo\/zh\/author\/emilychan\/","display_name":"emily chan"},"relative_dates":{"created":"Posted 3 \u5929 ago","modified":"Updated 3 \u5929 ago"},"absolute_dates":{"created":"Posted on 24 8 \u6708, 2026","modified":"Updated on 24 8 \u6708, 2026"},"absolute_dates_time":{"created":"Posted on 24 8 \u6708, 2026 5:24 \u4e0b\u5348","modified":"Updated on 24 8 \u6708, 2026 6:31 \u4e0b\u5348"},"featured_img_caption":"","tax_additional":{"tnc_tax_19993":{"linked":[],"unlinked":[],"slug":"tnc_tax_19993","name":"SDGs"},"tnc_tax_19818":{"linked":["ENGLISH<\/a>"],"unlinked":["ENGLISH<\/span>"],"slug":"tnc_tax_19818","name":"LANGUAGE"},"tnc_tax_19716":{"linked":["INSTITUTE OF SCIENCE AND ENVIRONMENT<\/a>"],"unlinked":["INSTITUTE OF SCIENCE AND ENVIRONMENT<\/span>"],"slug":"tnc_tax_19716","name":"FACULTY \/ RESEARCH UNIT"},"tnc_tax_19664":{"linked":[],"unlinked":[],"slug":"tnc_tax_19664","name":"SUBJECT"},"tnc_tax_19659":{"linked":["Journal Article<\/a>"],"unlinked":["Journal Article<\/span>"],"slug":"tnc_tax_19659","name":"Document Type"}},"series_order":"","_links":{"self":[{"href":"https:\/\/library.usj.edu.mo\/zh\/wp-json\/wp\/v2\/tnc_col_19837_item\/434558","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/library.usj.edu.mo\/zh\/wp-json\/wp\/v2\/tnc_col_19837_item"}],"about":[{"href":"https:\/\/library.usj.edu.mo\/zh\/wp-json\/wp\/v2\/types\/tnc_col_19837_item"}],"replies":[{"embeddable":true,"href":"https:\/\/library.usj.edu.mo\/zh\/wp-json\/wp\/v2\/comments?post=434558"}],"version-history":[{"count":1,"href":"https:\/\/library.usj.edu.mo\/zh\/wp-json\/wp\/v2\/tnc_col_19837_item\/434558\/revisions"}],"predecessor-version":[{"id":435345,"href":"https:\/\/library.usj.edu.mo\/zh\/wp-json\/wp\/v2\/tnc_col_19837_item\/434558\/revisions\/435345"}],"wp:attachment":[{"href":"https:\/\/library.usj.edu.mo\/zh\/wp-json\/wp\/v2\/media?parent=434558"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}